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buy doxycycline The genes were quantified from the purified DNA by real- time duplex PCR qPCR using the Qiagen s QuantiTect Multiplex NoROX Kit with the following conditions 10x HotStar Taq Polymerase buffer Qiagen, 200 ОјM dNTP, Primers LsFtsZ forward primer 5 cgatgagattatggaacatataa- 3, LsFtsZ reverse primer 5 ttgcaattactggtgctgc- 3, LsActin forward primer 5 atccaagctgtcctgtctct- 3, LsActin reverse primer 5 tgagaattgatttgagctaatg- 3, LsFtsZ taqman probe 5 6- FAM cagggatgggtggtggtactggaa 3 TAMRA, LsActin taqman probe 5 HEX actaccggtattgtgctcgatt 3 TAMRA, 2.

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